Molecular Diagnostics of Lung Cancer in Serous Effusion Samples

For molecular diagnostics of lung cancer samples, often only a small amount material is available. The ever-increasing number biomarker testing in contrast to the obtained. In that case, cytological specimens, such as serous effusion are one possible option. Effusion samples were prepared sediment smears or cytospins cell block if needed. Suitable tumor cells areas marked by cytopathologist and used for diagnostics, including fast track analysis, parallel sequencing, and/or fluorescence situ hybridization. 62 cases malignant with pulmonary adenocarcinoma, carried out. A fast-track result high-resolution melting method hotspot mutation KRAS Exon 2 EGFR exon 21 fragment length analysis 19 was available 43 out 47 (92%). Parallel sequencing successful 56 60 (93.3%). same period, 108 FISH analyses performed MET amplification, followed ROS1, RET, ALK translocation analysis. If limited tissue/biopsy available, advisable perform on high success rate.